buffer bio rad cat (Bio-Rad)
99
Structured Review
Bio-Rad
buffer bio rad cat
Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 12060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm41797917-294-120-121
Average 99 stars, based on 12060 article reviews
Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 12060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm41797917-294-120-121
Average 99 stars, based on 12060 article reviews
buffer bio rad cat - by Bioz Stars,
2026-09
99/100 stars
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Protease Inhibitor:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 Expressing:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 Plasmid Preparation:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 SYBR Green Assay:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 DNA Sequencing:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 Western Blot:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 RNA Sequencing:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 Mutagenesis:Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 Pore Size:Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Staining:Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% RNA sequencing:Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Gene Expression:Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Multiple Displacement Amplification:Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Sequencing:Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Recombinant:Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% |