Review



buffer bio rad cat  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Bio-Rad buffer bio rad cat
    Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 12060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm41797917-294-120-121
    Average 99 stars, based on 12060 article reviews
    buffer bio rad cat - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Protease Inhibitor:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    Expressing:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    Plasmid Preparation:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    SYBR Green Assay:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    DNA Sequencing:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    Western Blot:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    RNA Sequencing:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    Mutagenesis:

    Article Title: Sequential Regulation of Maternal mRNAs through a Conserved cis -Acting Element in Their 3′ UTRs
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFP abCam Cat# ab6556 Chicken polyclonal anti-GFP abCam Cat# ab13970 Rabbit polyclonal anti-pMad abCam Cat# ab52903 Mouse monoclonal anti-1B1 Developmental studies Hybridoma Bank Antibody Registry ID:528070 Rat monoclonal anti-HA high affinity Roche Diagnostics REF:11867423001 Rabbit polyclonal anti-Vasa Rangan Lab N/A Chicken polyclonal anti-Vasa Rangan Lab N/A Rabbit polyclonal anti-Pumilio Gift from Lehmann Lab N/A Rabbit polyclonal anti-Bruno Gift from Lehmann Lab N/A Rabbit polyclonal anti-Nanos Gift from Buszczak Lab N/A Rabbit polyclonal anti-Bruno Gift from Lily Lab (Sugimura and Lilly, 2006) N/A Anti-rabbit Alexa 488 Jackson ImmunoResearch Labs Code:711-546-152 Anti-chicken Alexa 488 Jackson ImmunoResearch Labs Code:703-546-155 Anti-rabbit Alexa Cy3 Jackson ImmunoResearch Labs Code:711-166-152 Anti-mouse Alexa Cy3 Jackson ImmunoResearch Labs Code:715-546-150 Anti-chicken Alexa 647 Jackson ImmunoResearch Labs Code:703-606-155 Anti-mouse Alexa 647 Jackson ImmunoResearch Labs Code:715-606-150 Anti-Rat HRP Jackson ImmunoResearch Labs Code:112-035-003 Anti-Rabbit HRP Jackson ImmunoResearch Labs Code:111-035-144 ChromePure Rabbit IgG Jackson ImmunoResearch Labs Code: 011-000-003 Bacterial and Virus Strains BL21(DE3) competent E.coli New England Biolabs Inc. Cat# C25271 KRX E.coli competent cells Promega Cat# L3002 DH5a competent cells Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins Formaldehyde (Methanol Free),10% Ultrapure Polysciences Inc. Cat# 04018-1 Donkey Serum Sigma-Aldrich SKU: D9663 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200 T4 Polynucleotide Kinase New England Biolabs Inc. Cat# M0201S Restriction Endonuclease XhoI New England Biolabs Inc. Cat# R0146S Restriction Endonuclease KpnI New England Biolabs Inc. Cat# R0142S Restriction Endonuclease AgeI New England Biolabs Inc. Cat# R0552S Restriction Endonuclease SpeI New England Biolabs Inc. Cat# R0133S Restriction Endonuclease NotI New England Biolabs Inc. Cat# R0189S Phusion High-Fidelity DNA Polymerase New England Biolabs Inc. Cat# M0530S HaloLink Resin Promega Cat# G1912 L-Rhamnose monohydrate Sigma-Aldrich SKU: R3875 IPTG Invitrogen Cat# 15529019 AcTEV Protease Invitrogen Cat# 12575015 LightShift Poly (dI-dC) ThermoFisher Scientific Cat# 20148E Yeast tRNA ThermoFisher Scientific Cat# AM7119 Salmon Sperm DNA ThermoFisher Scientific Cat# 15632011 (Continued on next page) e1 Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER Nonidet P-40 (NP-40) substitute IBI Scientific Cas# 9016-45-9 Tween-20 detergent VWR Cat# 97062-332 Triton X-100 detergent VWR Cat# 97062-208 Igepal CA-630 detergent Sigma-Aldrich SKU: I8896 DNase I Roche Cat# 04 716 728 001 Aprotinin Sigma-Aldrich SKU: 10236624001 PMSF Sigma-Aldrich SKU: 10837091001 Leupeptin protease inhibitor ThermoFisher Scientific Cat# 78435 Pepstatin A protease inhibitor ThermoFisher Scientific Cat# 78436 TRIzol Invitrogen Cat# 15596026 Dynabeads Protein A Invitrogen Cat# 10002D cOmplete, EDTA-free Protease Inhibitor Cocktail Pill Sigma-Aldrich SKU: 11873580001 Bradford reagent Bio-Rad Cat. #500-0205 4X Laemmli Sample Buffer Bio-Rad Cat. #161-0747 Ultrapure Sucrose Amresco Code: 0335-1KG Bruno expression plasmid pETM-82 EMBL (Chekulaeva et al., 2006) N/A Pumilio expression plasmid pFN18K Goldstrohm Lab (Weidmann et al., 2016) N/A Critical Commercial Assays G-25 Sephadix Columns Roche Cat# 11273990001 PD-10 column GE Health care Life Sciences Cat# 17-0851-01 His GraviTrap GE Health care Life Sciences Cat# 11-0033-99 TURBO DNA-free Kit Life Technologies Cat# AM1907 Super Script III Life Technologies Cat# 1808051 SYBR Green Master Mix Applied Biosystems Cat# 4367659 NEXTflex Rapid Illumina DNA-Seq Library Prep Kit Bioo Scientific Cat# NOVA-5138-11 Mini-PROTEAN TGX 4-20% gradient SDSPAGE gels Bio-Rad Cat# 456-1094 Western ECL Substrate Bio-Rad Cat# 1705060 Deposited Data RNA-seq Data This paper GEO: GSE119458 Polysome-seq Data This paper GEO: GSE119458 Experimental Models: Organisms/Strains D. melanogaster: w*; pgcD (Martinho et al., 2004; Flora et al., 2018) N/A D. melanogaster: w*; Df(2R)Liprin-gH1, P{neoFRT}42D Liprin-gH1/CyO Bloomington Drosophila Stock Center (Astigarraga et al., 2010) BDSC:63813; FlyBase: FBst0063813 D. melanogaster: w*; P{UAS-tkv.CA}3 Bloomington Drosophila Stock Center BDSC:36537; FlyBase: FBst0036537 D. melanogaster: pumFC8 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: pumET1 mutant (Forbes and Lehmann, 1998) N/A D. melanogaster: RNAi for pum: y1 v1; P{TRiP.JF02267}attP2 Bloomington Drosophila Stock Center BDSC:26725; FlyBase: FBst0026725 D. melanogaster: RNAi for pum: y1 sc* v1; P{TRiP.HMS01685}attP40 Bloomington Drosophila Stock Center BDSC:38241; FlyBase: FBst0038241 D. melanogaster: st1 pum680/TM3, Sb1 Ser1 Bloomington Drosophila Stock Center (Wharton et al., 1998) BDSC:3260; FlyBase: FBst0003260 D. melanogaster: nosRC mutant (Arrizabalaga and Lehmann, 1999) N/A D. melanogaster: nosBN mutant (Arrizabalaga and Lehmann, 1999) N/A (Continued on next page) Cell Reports 25, 3828–3843.e1–e9, December 26, 2018 e2 ..

    Pore Size:

    Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Bio-Rad Cat. # 1610183 Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit Bio-Rad Cat. # 1704271 4x Laemmli Sample Buffer Bio-Rad Cat. # 1610747 Deposited data Proteomic (collagen tracks) This paper https://www.ebi.ac.uk/pride/; ProteomeXchange: PXD035152 Bulk RNAseq (MCF-7) This paper https://www.ncbi.nlm.nih. gov/geo/; Gene Expression Omnibus: GSE308896 Bulk RNAseq (collagen tracks) This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies; ArrayExpress: E-MTAB-15636 Experimental models: Cell lines MDA-MB-231 ATCC Cat. # HTB-26 MCF-7 ATCC Cat. # HTB-22 MCF-10A ATCC Cat. # CRL-10317 Oligonucleotides Si ARN control5’UGGUUUACAUGUCGACUAA-3’ Eurofins N/A Si ARN β1 integrin #15’- GAACAGAUCUGAUGAAUGA-3’ Eurofins N/A Si ARN β1 integrin #35’- GAAGGGAGUUUGCUAAAUU-3’ Eurofins N/A Si ARN β1 integrin #45’- CCACAGACAUUUACAUUAA-3’ Eurofins N/A Si ARN CD44 #15’- GUAUGACACAUAUGGCUU-3’ Eurofins N/A Si ARN CD44 #25’- GAAGCAAUAUGUGUCAUA-3’ Eurofins N/A miRCURY LNA miRNA Power Inhibitors Qiagen See STAR Methods details for identifiers Primers for VIM,SNAI1, SNAI2, ZEB1, CDH1, DDR1, MMP14, ITGB1, 18S Eurofins See STAR Methods details for primer sequence Recombinant DNA crDNA to deplete DDR15’TGGGAAACACCGACCCTGCGGGG-3’ This paper N/A (Continued on next page) 18 Cell Reports 44, 116725, December 23, 2025 .. Anti-DDR1 (5583S), anti-CD9 (13174) and anti-clathrin light chain (4796S) were purchased from Cell Signaling.

    Staining:

    Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Bio-Rad Cat. # 1610183 Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit Bio-Rad Cat. # 1704271 4x Laemmli Sample Buffer Bio-Rad Cat. # 1610747 Deposited data Proteomic (collagen tracks) This paper https://www.ebi.ac.uk/pride/; ProteomeXchange: PXD035152 Bulk RNAseq (MCF-7) This paper https://www.ncbi.nlm.nih. gov/geo/; Gene Expression Omnibus: GSE308896 Bulk RNAseq (collagen tracks) This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies; ArrayExpress: E-MTAB-15636 Experimental models: Cell lines MDA-MB-231 ATCC Cat. # HTB-26 MCF-7 ATCC Cat. # HTB-22 MCF-10A ATCC Cat. # CRL-10317 Oligonucleotides Si ARN control5’UGGUUUACAUGUCGACUAA-3’ Eurofins N/A Si ARN β1 integrin #15’- GAACAGAUCUGAUGAAUGA-3’ Eurofins N/A Si ARN β1 integrin #35’- GAAGGGAGUUUGCUAAAUU-3’ Eurofins N/A Si ARN β1 integrin #45’- CCACAGACAUUUACAUUAA-3’ Eurofins N/A Si ARN CD44 #15’- GUAUGACACAUAUGGCUU-3’ Eurofins N/A Si ARN CD44 #25’- GAAGCAAUAUGUGUCAUA-3’ Eurofins N/A miRCURY LNA miRNA Power Inhibitors Qiagen See STAR Methods details for identifiers Primers for VIM,SNAI1, SNAI2, ZEB1, CDH1, DDR1, MMP14, ITGB1, 18S Eurofins See STAR Methods details for primer sequence Recombinant DNA crDNA to deplete DDR15’TGGGAAACACCGACCCTGCGGGG-3’ This paper N/A (Continued on next page) 18 Cell Reports 44, 116725, December 23, 2025 .. Anti-DDR1 (5583S), anti-CD9 (13174) and anti-clathrin light chain (4796S) were purchased from Cell Signaling.

    RNA sequencing:

    Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Bio-Rad Cat. # 1610183 Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit Bio-Rad Cat. # 1704271 4x Laemmli Sample Buffer Bio-Rad Cat. # 1610747 Deposited data Proteomic (collagen tracks) This paper https://www.ebi.ac.uk/pride/; ProteomeXchange: PXD035152 Bulk RNAseq (MCF-7) This paper https://www.ncbi.nlm.nih. gov/geo/; Gene Expression Omnibus: GSE308896 Bulk RNAseq (collagen tracks) This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies; ArrayExpress: E-MTAB-15636 Experimental models: Cell lines MDA-MB-231 ATCC Cat. # HTB-26 MCF-7 ATCC Cat. # HTB-22 MCF-10A ATCC Cat. # CRL-10317 Oligonucleotides Si ARN control5’UGGUUUACAUGUCGACUAA-3’ Eurofins N/A Si ARN β1 integrin #15’- GAACAGAUCUGAUGAAUGA-3’ Eurofins N/A Si ARN β1 integrin #35’- GAAGGGAGUUUGCUAAAUU-3’ Eurofins N/A Si ARN β1 integrin #45’- CCACAGACAUUUACAUUAA-3’ Eurofins N/A Si ARN CD44 #15’- GUAUGACACAUAUGGCUU-3’ Eurofins N/A Si ARN CD44 #25’- GAAGCAAUAUGUGUCAUA-3’ Eurofins N/A miRCURY LNA miRNA Power Inhibitors Qiagen See STAR Methods details for identifiers Primers for VIM,SNAI1, SNAI2, ZEB1, CDH1, DDR1, MMP14, ITGB1, 18S Eurofins See STAR Methods details for primer sequence Recombinant DNA crDNA to deplete DDR15’TGGGAAACACCGACCCTGCGGGG-3’ This paper N/A (Continued on next page) 18 Cell Reports 44, 116725, December 23, 2025 .. Anti-DDR1 (5583S), anti-CD9 (13174) and anti-clathrin light chain (4796S) were purchased from Cell Signaling.

    Gene Expression:

    Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Bio-Rad Cat. # 1610183 Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit Bio-Rad Cat. # 1704271 4x Laemmli Sample Buffer Bio-Rad Cat. # 1610747 Deposited data Proteomic (collagen tracks) This paper https://www.ebi.ac.uk/pride/; ProteomeXchange: PXD035152 Bulk RNAseq (MCF-7) This paper https://www.ncbi.nlm.nih. gov/geo/; Gene Expression Omnibus: GSE308896 Bulk RNAseq (collagen tracks) This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies; ArrayExpress: E-MTAB-15636 Experimental models: Cell lines MDA-MB-231 ATCC Cat. # HTB-26 MCF-7 ATCC Cat. # HTB-22 MCF-10A ATCC Cat. # CRL-10317 Oligonucleotides Si ARN control5’UGGUUUACAUGUCGACUAA-3’ Eurofins N/A Si ARN β1 integrin #15’- GAACAGAUCUGAUGAAUGA-3’ Eurofins N/A Si ARN β1 integrin #35’- GAAGGGAGUUUGCUAAAUU-3’ Eurofins N/A Si ARN β1 integrin #45’- CCACAGACAUUUACAUUAA-3’ Eurofins N/A Si ARN CD44 #15’- GUAUGACACAUAUGGCUU-3’ Eurofins N/A Si ARN CD44 #25’- GAAGCAAUAUGUGUCAUA-3’ Eurofins N/A miRCURY LNA miRNA Power Inhibitors Qiagen See STAR Methods details for identifiers Primers for VIM,SNAI1, SNAI2, ZEB1, CDH1, DDR1, MMP14, ITGB1, 18S Eurofins See STAR Methods details for primer sequence Recombinant DNA crDNA to deplete DDR15’TGGGAAACACCGACCCTGCGGGG-3’ This paper N/A (Continued on next page) 18 Cell Reports 44, 116725, December 23, 2025 .. Anti-DDR1 (5583S), anti-CD9 (13174) and anti-clathrin light chain (4796S) were purchased from Cell Signaling.

    Multiple Displacement Amplification:

    Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Bio-Rad Cat. # 1610183 Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit Bio-Rad Cat. # 1704271 4x Laemmli Sample Buffer Bio-Rad Cat. # 1610747 Deposited data Proteomic (collagen tracks) This paper https://www.ebi.ac.uk/pride/; ProteomeXchange: PXD035152 Bulk RNAseq (MCF-7) This paper https://www.ncbi.nlm.nih. gov/geo/; Gene Expression Omnibus: GSE308896 Bulk RNAseq (collagen tracks) This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies; ArrayExpress: E-MTAB-15636 Experimental models: Cell lines MDA-MB-231 ATCC Cat. # HTB-26 MCF-7 ATCC Cat. # HTB-22 MCF-10A ATCC Cat. # CRL-10317 Oligonucleotides Si ARN control5’UGGUUUACAUGUCGACUAA-3’ Eurofins N/A Si ARN β1 integrin #15’- GAACAGAUCUGAUGAAUGA-3’ Eurofins N/A Si ARN β1 integrin #35’- GAAGGGAGUUUGCUAAAUU-3’ Eurofins N/A Si ARN β1 integrin #45’- CCACAGACAUUUACAUUAA-3’ Eurofins N/A Si ARN CD44 #15’- GUAUGACACAUAUGGCUU-3’ Eurofins N/A Si ARN CD44 #25’- GAAGCAAUAUGUGUCAUA-3’ Eurofins N/A miRCURY LNA miRNA Power Inhibitors Qiagen See STAR Methods details for identifiers Primers for VIM,SNAI1, SNAI2, ZEB1, CDH1, DDR1, MMP14, ITGB1, 18S Eurofins See STAR Methods details for primer sequence Recombinant DNA crDNA to deplete DDR15’TGGGAAACACCGACCCTGCGGGG-3’ This paper N/A (Continued on next page) 18 Cell Reports 44, 116725, December 23, 2025 .. Anti-DDR1 (5583S), anti-CD9 (13174) and anti-clathrin light chain (4796S) were purchased from Cell Signaling.

    Sequencing:

    Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Bio-Rad Cat. # 1610183 Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit Bio-Rad Cat. # 1704271 4x Laemmli Sample Buffer Bio-Rad Cat. # 1610747 Deposited data Proteomic (collagen tracks) This paper https://www.ebi.ac.uk/pride/; ProteomeXchange: PXD035152 Bulk RNAseq (MCF-7) This paper https://www.ncbi.nlm.nih. gov/geo/; Gene Expression Omnibus: GSE308896 Bulk RNAseq (collagen tracks) This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies; ArrayExpress: E-MTAB-15636 Experimental models: Cell lines MDA-MB-231 ATCC Cat. # HTB-26 MCF-7 ATCC Cat. # HTB-22 MCF-10A ATCC Cat. # CRL-10317 Oligonucleotides Si ARN control5’UGGUUUACAUGUCGACUAA-3’ Eurofins N/A Si ARN β1 integrin #15’- GAACAGAUCUGAUGAAUGA-3’ Eurofins N/A Si ARN β1 integrin #35’- GAAGGGAGUUUGCUAAAUU-3’ Eurofins N/A Si ARN β1 integrin #45’- CCACAGACAUUUACAUUAA-3’ Eurofins N/A Si ARN CD44 #15’- GUAUGACACAUAUGGCUU-3’ Eurofins N/A Si ARN CD44 #25’- GAAGCAAUAUGUGUCAUA-3’ Eurofins N/A miRCURY LNA miRNA Power Inhibitors Qiagen See STAR Methods details for identifiers Primers for VIM,SNAI1, SNAI2, ZEB1, CDH1, DDR1, MMP14, ITGB1, 18S Eurofins See STAR Methods details for primer sequence Recombinant DNA crDNA to deplete DDR15’TGGGAAACACCGACCCTGCGGGG-3’ This paper N/A (Continued on next page) 18 Cell Reports 44, 116725, December 23, 2025 .. Anti-DDR1 (5583S), anti-CD9 (13174) and anti-clathrin light chain (4796S) were purchased from Cell Signaling.

    Recombinant:

    Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen tracks.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Human TSPAN4-GFP plasmid GenScript NM_001025239.1 Chemicals, peptides, and recombinant proteins Hoechst Interchim Cat. #H21486 Phalloidin Interchim Cat. # FP-BZ-9630 5-carboxy-X-rhodamine succinimidyl ester Interchim Cat. # FP-68336A Cell Mask Invitrogen Cat. #C10046 Type I collagen Corning Cat. # 354236 Type III collagen CellSystems Cat. # 5021 Fibronectin Sigma Aldrich Cat. #F1056 Matrigel Dutcher Cat. # 356231 Manganese Sigma Aldrich Cat. #M7899-500G Dapi Sigma Aldrich Cat. #D9542 GM6001 Sigma Aldrich Cat. # CC10 LOX-L2 R&D system Cat. # 2639-AO Gelatin Sigma Aldrich Cat. #G1393 Cytochalasin D Sigma Aldrich Cat. #C2618 Blebbistatin Sigma Aldrich Cat. #B0560 DMSO Sigma Aldrich Cat. #D8418 Acid acetic Merck Cat. # 100063 Glutaraldehyde Electron Microscopy Sciences Cat. # 16000 PFA 4% in PBS Clinisciences Cat. # sc-281692 Pirfenidone MedchemExpress Cat. # HY-B0673 EGF Sigma Aldrich Cat. #E1235 Cholera toxin Sigma Aldrich Cat. #C8052 Hydrocortisone Sigma Aldrich Cat. #H0888 Insulin Sigma Aldrich Cat. #I3536 EDTA Euromedex Cat. # EU0084 Triton X-100 Sigma Aldrich Cat. #T9284 BSA Sigma Aldrich Cat. # 05470 Fluoromount-G mounting media SouthernBiotech Cat. # 0100-01 Phosphate Buffer Electron Microscopy Sciences Cat. # 50-365-831 Complete Roche Cat. # 04693116001 PhosStop Roche Cat. # PHOSS-RO TBS 10X Euromedex Cat. # ET220 Tween Sigma Aldrich Cat. #P7949 SYBR® Green SuperMix for iQTM Quanta Biosciences, Inc Cat. # 95053-500 Lipofectamine RNAimax Invitrogen Cat. # 13778150 Oregon Green TM 488 Conjugate Thermofisher Cat. #G13186 Trizol Invitrogen Cat. # 15596026 Maxima Reverse Transcriptase Thermofisher Cat. # 10869200 PageRuler TM Plus Prestained Protein Ladder, 10 to 250 kDa Thermo Scientific Cat. # 26620 Ripa Merck Cat. # 20-188 EveryBlot Blocking Buffer Bio Rad Cat. # 12010020 lipofectamine RNAimax Invitrogen Cat. # 13778150 RNASE A Sigma Aldrich Cat. #R6148 (Continued on next page) Cell Reports 44, 116725, December 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FBS Eurobio Cat. # CVFSVF00-01 Horse serum Gibco Cat. # 16050122 DMEM high glucose, Glutamax Gibco Cat. # 10566016 DMEM-F12, Glutamax Gibco Cat. # 10565018 RPMI, Glutamax Gibco Cat. # 61870036 Penicillin-streptomycin Gibco Cat. # 15140122 Trypsin Gibco Cat. # 25300054 DPBS Gibco Cat. # 14190144 PBS Gibco Cat. # 10010023 OptiMEM Gibco Cat. # 11058021 Nucleospin RNA kit Macherey Nagel Cat. # 740955.50 Transwell chambers 8.0μm pore size Falcon Cat. # 353097 TGX Stain-Free TM FastCast TM Acrylamide Kit, 10% Bio-Rad Cat. # 1610183 Trans-Blot Turbo RTA Midi 0.2 μm Nitrocellulose Transfer Kit Bio-Rad Cat. # 1704271 4x Laemmli Sample Buffer Bio-Rad Cat. # 1610747 Deposited data Proteomic (collagen tracks) This paper https://www.ebi.ac.uk/pride/; ProteomeXchange: PXD035152 Bulk RNAseq (MCF-7) This paper https://www.ncbi.nlm.nih. gov/geo/; Gene Expression Omnibus: GSE308896 Bulk RNAseq (collagen tracks) This paper https://www.ebi.ac.uk/biostudies/ arrayexpress/studies; ArrayExpress: E-MTAB-15636 Experimental models: Cell lines MDA-MB-231 ATCC Cat. # HTB-26 MCF-7 ATCC Cat. # HTB-22 MCF-10A ATCC Cat. # CRL-10317 Oligonucleotides Si ARN control5’UGGUUUACAUGUCGACUAA-3’ Eurofins N/A Si ARN β1 integrin #15’- GAACAGAUCUGAUGAAUGA-3’ Eurofins N/A Si ARN β1 integrin #35’- GAAGGGAGUUUGCUAAAUU-3’ Eurofins N/A Si ARN β1 integrin #45’- CCACAGACAUUUACAUUAA-3’ Eurofins N/A Si ARN CD44 #15’- GUAUGACACAUAUGGCUU-3’ Eurofins N/A Si ARN CD44 #25’- GAAGCAAUAUGUGUCAUA-3’ Eurofins N/A miRCURY LNA miRNA Power Inhibitors Qiagen See STAR Methods details for identifiers Primers for VIM,SNAI1, SNAI2, ZEB1, CDH1, DDR1, MMP14, ITGB1, 18S Eurofins See STAR Methods details for primer sequence Recombinant DNA crDNA to deplete DDR15’TGGGAAACACCGACCCTGCGGGG-3’ This paper N/A (Continued on next page) 18 Cell Reports 44, 116725, December 23, 2025 .. Anti-DDR1 (5583S), anti-CD9 (13174) and anti-clathrin light chain (4796S) were purchased from Cell Signaling.



    Similar Products

    99
    Bio-Rad buffer bio rad cat
    Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm41797917-294-120-121
    Average 99 stars, based on 1 article reviews
    buffer bio rad cat - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad 4x laemmli sample buffer bio rad cat
    4x Laemmli Sample Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm41379613-619-106-110
    Average 99 stars, based on 1 article reviews
    4x laemmli sample buffer bio rad cat - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad v v ethanol 4 × laemmli buffer bio rad cat no 1610747
    V V Ethanol 4 × Laemmli Buffer Bio Rad Cat No 1610747, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm37166238-189-49-54
    Average 99 stars, based on 1 article reviews
    v v ethanol 4 × laemmli buffer bio rad cat no 1610747 - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad bio rad cat
    Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm36135312-78-27-27
    Average 99 stars, based on 1 article reviews
    bio rad cat - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad e0552s 4x laemmli sample buffer bio rad cat
    E0552s 4x Laemmli Sample Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm35762422-402-162-167
    Average 99 stars, based on 1 article reviews
    e0552s 4x laemmli sample buffer bio rad cat - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad p35g 1 0 14 c 4x laemmli sample buffer bio rad cat
    P35g 1 0 14 C 4x Laemmli Sample Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm33503419-174-44-49
    Average 99 stars, based on 1 article reviews
    p35g 1 0 14 c 4x laemmli sample buffer bio rad cat - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad a 405 4x laemmli sample buffer bio rad
    A 405 4x Laemmli Sample Buffer Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm33657368-227-116-121
    Average 99 stars, based on 1 article reviews
    a 405 4x laemmli sample buffer bio rad - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad bio rad cat 1610747
    Bio Rad Cat 1610747, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pm33417860-698-39-39
    Average 99 stars, based on 1 article reviews
    bio rad cat 1610747 - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad laemmli buffer bio rad cat
    Laemmli Buffer Bio Rad Cat, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+sample+buffer+bio+rad+cat/4x+Laemmli+Sample+Buffer/pmc07003067__mmc5-333-156-158
    Average 99 stars, based on 1 article reviews
    laemmli buffer bio rad cat - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results